In vitro development of human primordial follicles to preantral stage after vitrification
نویسندگان
چکیده
منابع مشابه
P-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...
متن کاملIn vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification.
The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, d...
متن کاملVitrification and Subsequent In Vitro Maturation of Mouse Preantral Follicles in Presence of Growth Factors
OBJECTIVE Cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. Although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and epidermal growth factor (EGF) on in vitro prea...
متن کاملActivin promotes oocyte development in ovine preantral follicles in vitro
Activins have been implicated as important regulating factors for many reproductive processes. The aim of this study was to determine the effect of activin A on the development of ovine preantral follicles in vitro. Mechanically isolated preantral follicles (161 +/- 2 microm) were cultured for 6 days in the presence of human recombinant activin A (0, 10 and 100 ng/ml). Half of the medium was re...
متن کاملIn vitro culture of goat preantral follicles
Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2013
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-013-0105-z